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PDE12 Polyclonal antibody

PDE12 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12753-1-AP

Print datasheet

Synonyms

2 PDE, 2' PDE, 2,5 phosphodiesterase 12, PDE12, phosphodiesterase 12

验证数据展示

  • WB analysis of human brain using 12753-1-AP

    WB analysis of human brain using 12753-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12753-1-AP

    IF Staining of HepG2 using 12753-1-AP

    Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse brain using 12753-1-AP

    IP experiment of mouse brain using 12753-1-AP

    IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman brain tissue, THP-1 cells
Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12753-1-AP targets PDE12 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen PDE12 fusion protein Ag3217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphodiesterase 12
Synonyms 2 PDE, 2' PDE, 2,5 phosphodiesterase 12, PDE12, phosphodiesterase 12
Calculated Molecular Weight 472 aa, 52 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC034978
Gene Symbol PDE12
Gene ID (NCBI) 201626
RRIDAB_2162883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6L8Q7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDE12 antibody 12753-1-APDownload protocol
IF protocol for PDE12 antibody 12753-1-APDownload protocol
IP protocol for PDE12 antibody 12753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols