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验证数据展示

  • WB analysis using 17407-1-AP

    WB analysis using 17407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17407-1-AP (PDCL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 17407-1-AP

    WB analysis of mouse testis using 17407-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17407-1-AP (PDCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17407-1-AP

    IHC staining of human kidney using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 17407-1-AP

    IHC staining of human kidney using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

PDCL2 Polyclonal antibody

PDCL2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17407-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

GCPHLP, PDCL2, phosducin like 2, Phosducin like protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17407-1-AP targets PDCL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 PDCL2 fusion protein Ag11427 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosducin-like 2
别名 GCPHLP, PDCL2, phosducin like 2, Phosducin like protein 2
计算分子量 250 aa, 29 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC034431
基因名称 PDCL2
Gene ID (NCBI) 132954
RRIDAB_2299492
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N4E4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDCL2 antibody 17407-1-APDownload protocol
IHC protocol for PDCL2 antibody 17407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Andrology

PDCL2 is essential for sperm acrosome formation and male fertility in mice

Authors - Yoshitaka Fujihara
View Article
humanIHC

Transl Androl Urol

Identification of PDCL2 as a candidate marker in Sertoli cell-only syndrome by chromatin immunoprecipitation-sequencing and bioinformatics analysis

Authors - Yu Yang
View Article
mouseWB

PNAS Nexus

Tex46 knockout male mice are sterile secondary to sperm head malformations and failure to penetrate through the zona pellucida

Authors - Yoshitaka Fujihara
View Article