• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85792-5-RR

    WB analysis using 85792-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85792-5-RR (PDCD6/ALG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 85792-5-RR

    WB analysis of HeLa using 85792-5-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85792-5-RR (PDCD6/ALG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PDCD6/ALG2 Recombinant antibody

PDCD6/ALG2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85792-5-RR

产品说明书

CloneNo. 250087B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

ALG2, PDCD6, ALG 2, ALG-2, Apoptosis-linked gene 2 protein homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, K-562 cells, SK-BR-3 cells, NIH/3T3 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy PDCD6 Ready-To-Use IHC Kit. PDCD6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85792-5-RR targets PDCD6/ALG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PDCD6/ALG2 fusion protein Ag2949 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 programmed cell death 6
别名 ALG2, PDCD6, ALG 2, ALG-2, Apoptosis-linked gene 2 protein homolog
计算分子量 191 aa, 22 kDa
观测分子量 15-22 kDa
GenBank蛋白编号BC012384
基因名称 PDCD6
Gene ID (NCBI) 10016
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75340
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death protein 6 (PDCD6) is also named as ALG2. PDCD6 is an evolutionarily conserved Ca2+-binding protein. PDCD6 is required for coat protein complex II-dependent endoplasmic reticulum-to-Golgi apparatus vesicular transport in the cytoplasm (PMID: 39934130). PDCD6 is involved in regulating multifaceted and pleiotropic cellular processes in different cellular compartments. The nuclear PDCD6 regulates apoptosis and alternative splicing. And cytoplasmic PDCD6 is involved in the regulation of cytoskeletal dynamics and innate immune responses. Additionally, membranous PDCD6 participates in membrane repair through endosomal sorting complex required for transport complex-dependent membrane budding. (PMID: 38436472). PDCD6 also acts as a regulator of cytosolic DNA-induced immune responses by modulating STING transport (PMID: 34787301).

实验方案

Product Specific Protocols
WB protocol for PDCD6/ALG2 antibody 85792-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols