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验证数据展示

  • WB analysis of HEK293 cells using 66100-1-Ig (same clone as 66100-1-PBS)

    WB analysis of HEK293 cells using 66100-1-Ig (same clone as 66100-1-PBS)

    WB result of PDCD4 antibody (66100-1-Ig, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDCD4 antibody included
  • WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

    WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDCD4 antibody included
  • Featured Product
  • KD/KO验证

PDCD4 Monoclonal antibody, PBS Only

PDCD4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66100-1-PBS

产品说明书

CloneNo. 1F1A11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PDCD4 Ready-To-Use IHC Kit. PDCD4 primary antibody included.

产品信息

66100-1-PBS targets PDCD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 PDCD4 fusion protein Ag19239 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 4 (neoplastic transformation inhibitor)
别名 H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a
计算分子量 469 aa, 52 kDa
观测分子量 56 kDa
GenBank蛋白编号BC026104
基因名称 PDCD4
Gene ID (NCBI) 27250
RRIDAB_2881499
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ53EL6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.