验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MDA-MB-468 cells, MRC-5 cells, HuH-7 cells, PANC-1 cells, MCF-7 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87870-1-RR targets PDAP1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag7155 Product name: Recombinant human PDAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC000684 Sequence: MPKGGRKGGHKGRARQYTSPEEIDAQLQAEKQKAREEEEQKEGGDGAAGDPKKEKKSLDSDESEDEEDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLDLDGPKELSRREREEIEKQKAKERYMKMHLAGKTEQAKADLARLAIIRKQREEAARKKEEERKAKDDATLSGKRMQSLSLNK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | PDGFA associated protein 1 |
| 别名 | 28 kDa heat- and acid-stable phosphoprotein, HASPP28, PDGF associated protein, PDGFA associated protein 1, PDGFA-associated protein 1 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC000684 |
| 基因名称 | PDAP1 |
| Gene ID (NCBI) | 11333 |
| RRID | AB_3745798 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13442 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDAP1 (PDGFA-associated protein 1), also known as PAP, PAP1, or HASPP28 (28 kDa heat- and acid-stable phosphoprotein), is a protein-coding gene located on human chromosome 7q22.1 . The gene consists of 6 exons and encodes a 28 kDa phosphoprotein that is highly conserved across vertebrate species.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDAP1 antibody 87870-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
