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验证数据展示

  • Sandwich ELISA standard curve of MP00844-2

    Sandwich ELISA standard curve of MP00844-2

    Sandwich ELISA standard curve of MP00844-2, Mouse PD-L1/CD274 Recombinant Matched Antibody Pair - PBS only. 83906-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0986. 83906-1-PBS was HRP conjugated as the detection antibody. Range: 4.69-300 pg/mL

  • Sample test of MP00844-2

    Sample test of MP00844-2

    RAW264.7 and NIH/3T3 cell lysate were measured. The mouse PD-L1/CD274 concentration was determined to be 918.7 pg/mL in RAW264.7 cell lysate (based on a 4.8 mg/mL extract load) and 144.6 pg/mL in NIH/3T3 cell lysate (based on a 10.6 mg/mL extract load).

  • Sample test of MP00844-2

    Sample test of MP00844-2

    Serum of sixteen individual healthy human donors was measured. The mouse PD-L1/CD274 concentration of detected samples was determined to be 99.6 pg/mL with a range of 40.12 - 315.13 pg/mL.

  • Cytometric bead array standard curve of MP00844-2

    Cytometric bead array standard curve of MP00844-2

    Cytometric bead array standard curve of MP00844-2, MOUSE PD-L1/CD274 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83906-4-PBS. Detection antibody: 83906-1-PBS. Standard: Eg0986. Range: 0.313-40 ng/mL.

Mouse PD-L1/CD274 Recombinant antibody, PBS Only (Capture)

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83906-4-PBS

产品说明书

CloneNo. 240993H1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83906-4-PBS targets PD-L1/CD274 as part of a matched antibody pair:

MP00844-2: 83906-4-PBS capture and 83906-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD274 antigen
别名 Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1
计算分子量33 kDa
GenBank蛋白编号NM_021893.3
基因名称 PD-L1
Gene ID (NCBI) 60533
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9EP73
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C