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验证数据展示

  • WB analysis using 66220-1-Ig

    WB analysis using 66220-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IF Staining of human tonsillitis using 66220-1-Ig

    IF Staining of human tonsillitis using 66220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

  • IF Staining of human tonsillitis using 66220-1-Ig

    IF Staining of human tonsillitis using 66220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using PD-1/CD279 antibody (66220-1-Ig, Clone: 4H4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 66220-1-Ig

    IHC staining of human appendicitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 66220-1-Ig

    IHC staining of human lymphoma using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 66220-1-Ig

    IHC staining of human lymphoma using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 66220-1-Ig

    IHC staining of human appendicitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human lung cancer using 66220-1-Ig

    IHC staining of human lung cancer using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human breast cancer using 66220-1-Ig

    IHC staining of human breast cancer using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human lymphoma using 66220-1-Ig

    IHC staining of human lymphoma using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human lymphoma using 66220-1-Ig

    IHC staining of human lymphoma using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human appendicitis using 66220-1-Ig

    IHC staining of human appendicitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • Featured Product
  • KD/KO Validated

PD-1/CD279 Monoclonal antibody

PD-1/CD279 Monoclonal Antibody for WB, IHC, IHC-Autostainer, IF-P, ELISA
Cat No. 66220-1-Ig

产品说明书

CloneNo. 4H4D1
引用文献(112)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IHC-Autostainer, IF-P, ELISA and More (2)

别名

CD279, PD1, PD-1, 4H4D1, hPD 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue
Positive IHC-Autostainer detected inhuman appendicitis tissue, human lymphoma tissue, human tonsillitis tissue
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human lung cancer tissue, human lymphoma tissue, human tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human lymphoma tissue, mouse spleen tissue, mouse thymus tissue
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:10000-1:40000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66220-1-Ig targets PD-1/CD279 in WB, IHC, IHC-Autostainer, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IHC-Autostainer, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag12470

Product name: Recombinant human PD-1/CD279 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-288 aa of BC074740

Sequence: MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGSLVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVPCVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 1
别名 CD279, PD1, PD-1, 4H4D1, hPD 1
计算分子量 288 aa, 32 kDa
观测分子量 32 kDa, 47-55 kDa
GenBank蛋白编号BC074740
基因名称 PD-1
Gene ID (NCBI) 5133
RRIDAB_2881611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15116
储存缓冲液PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

实验方案

Product Specific Protocols
IF protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
IHC protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
WB protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Signal Transduct Target Ther

Chemo-photothermal synergy ignites antitumor immunity via ferroptosis.

Authors - Jundong Lin
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humanIF

Cell

Systematic profiling reveals betaine as an exercise mimetic for geroprotection

Authors - Lingling Geng
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mouseIF

Cell Metab

Hepatic acetyl-CoA metabolism modulates neuroinflammation and depression susceptibility via acetate

Authors - Yu Cao
View Article
mouseWB,IHC

Cell Metab

Macrophage PD-1 regulates energy expenditure and metabolic dysfunction under immune checkpoint blockade.

Authors - Ming-Ming Wu
  • KO Validated
View Article
humanWB,IF

Cancer Commun (Lond)

Metabolic landscape of head and neck squamous cell carcinoma informs a novel kynurenine/Siglec-15 axis in immune escape

Authors - Xin-Yu Zhang
View Article
mouseIF

Nat Genet

Single-cell multi-omic and spatial profiling of esophageal squamous cell carcinoma reveals the immunosuppressive role of GPR116+ pericytes in cancer metastasis

Authors - Xiaoya Pei
View Article
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