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验证数据展示

  • WB analysis using 66220-1-Ig

    WB analysis using 66220-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IF Staining of human tonsillitis using 66220-1-Ig

    IF Staining of human tonsillitis using 66220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

  • IF Staining of human tonsillitis using 66220-1-Ig

    IF Staining of human tonsillitis using 66220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using PD-1/CD279 antibody (66220-1-Ig, Clone: 4H4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 66220-1-Ig

    IHC staining of human appendicitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 66220-1-Ig

    IHC staining of human lymphoma using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 66220-1-Ig

    IHC staining of human lymphoma using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 66220-1-Ig

    IHC staining of human appendicitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human lung cancer using 66220-1-Ig

    IHC staining of human lung cancer using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human breast cancer using 66220-1-Ig

    IHC staining of human breast cancer using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human lymphoma using 66220-1-Ig

    IHC staining of human lymphoma using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human lymphoma using 66220-1-Ig

    IHC staining of human lymphoma using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human tonsillitis using 66220-1-Ig

    IHC staining of human tonsillitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • IHC staining of human appendicitis using 66220-1-Ig

    IHC staining of human appendicitis using 66220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PD-1 antibody included
  • Featured Product
  • KD/KO Validated

PD-1/CD279 Monoclonal antibody

PD-1/CD279 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66220-1-Ig

产品说明书

CloneNo. 4H4D1
引用文献(106)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA and More (2)

别名

CD279, PD1, PD-1, 4H4D1, hPD 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human lymphoma tissue, mouse spleen tissue, mouse thymus tissue
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66220-1-Ig targets PD-1/CD279 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag12470

Product name: Recombinant human PD-1/CD279 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-288 aa of BC074740

Sequence: MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGSLVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVPCVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 1
别名 CD279, PD1, PD-1, 4H4D1, hPD 1
计算分子量 288 aa, 32 kDa
观测分子量 32 kDa, 47-55 kDa
GenBank蛋白编号BC074740
基因名称 PD-1
Gene ID (NCBI) 5133
RRIDAB_2881611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15116
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

实验方案

Product Specific Protocols
IF protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
IHC protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
WB protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Signal Transduct Target Ther

Chemo-photothermal synergy ignites antitumor immunity via ferroptosis.

Authors - Jundong Lin
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humanIF

Cell

Systematic profiling reveals betaine as an exercise mimetic for geroprotection

Authors - Lingling Geng
View Article
mouseIF

Cell Metab

Hepatic acetyl-CoA metabolism modulates neuroinflammation and depression susceptibility via acetate

Authors - Yu Cao
View Article
mouseWB,IHC

Cell Metab

Macrophage PD-1 regulates energy expenditure and metabolic dysfunction under immune checkpoint blockade.

Authors - Ming-Ming Wu
  • KO Validated
View Article
humanWB,IF

Cancer Commun (Lond)

Metabolic landscape of head and neck squamous cell carcinoma informs a novel kynurenine/Siglec-15 axis in immune escape

Authors - Xin-Yu Zhang
View Article
mouseIF

Nat Genet

Single-cell multi-omic and spatial profiling of esophageal squamous cell carcinoma reveals the immunosuppressive role of GPR116+ pericytes in cancer metastasis

Authors - Xiaoya Pei
View Article
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