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验证数据展示

  • WB analysis using 68355-1-Ig

    WB analysis using 68355-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68355-1-Ig (PCYT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68355-1-Ig

    IF Staining of HepG2 using 68355-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCYT2 antibody (68355-1-Ig, Clone: 2F7G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PCYT2 Monoclonal antibody

PCYT2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68355-1-Ig

产品说明书

CloneNo. 2F7G1

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

ET, PCYT2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68355-1-Ig targets PCYT2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag6604

Product name: Recombinant human PCYT2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-389 aa of BC000351

Sequence: MIRNGRGAAGGAEQPGPGGRRAVRVWCDGCYDMVHYGHSNQLRQARAMGDYLIVGVHTDEEIAKHKGPPVFTQEERYKMVQAIKWVDEVVPAAPYVTTLETLDKYNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKRTQGVSTTDLVGRMLLVTKAHHSSQEMSSEYREYADSFGKCPGGRNPWTGVSQFLQTSQKIIQFASGKEPQPGETVIYVAGAFDLFHIGHVDFLEKVHRLAERPYIIAGLHFDQEVNHYKGKNYPIMNLHERTLSVLACRYVSEVVIGAPYAVTAELLSHFKVDLVCHGKTEIIPDRDGSDPYQEPKRRGIFRQIDSGSNLTTDLIVQRIITNRLEYEARNQKKEAKELAFLEAARQQAAQPLGERDGDF

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphate cytidylyltransferase 2, ethanolamine
别名 ET, PCYT2
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC000351
基因名称 PCYT2
Gene ID (NCBI) 5833
RRIDAB_3085077
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99447
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ethanolamine-phosphate cytidylyltransferase (PCYT2) catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PMID:9083101, PMID:31637422). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PMID:9083101).

实验方案

Product Specific Protocols
IF protocol for PCYT2 antibody 68355-1-IgDownload protocol
WB protocol for PCYT2 antibody 68355-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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