验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, human brain tissue |
| Positive IP detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13765-1-AP targets PCYT1B/PCYT1A in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | PCYT1B/PCYT1A fusion protein Ag4748 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphate cytidylyltransferase 1, choline, beta |
| 别名 | PCYT1B, CCT B, CCT beta, CCTB, CCT-beta |
| 计算分子量 | 351 aa, 40 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC045634 |
| 基因名称 | PCYT1B |
| Gene ID (NCBI) | 9468 |
| RRID | AB_10642953 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5K3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCYT1B (Phosphate Cytidylyltransferase 1B, Choline), also named as CTβ, CCTB and CCT-Beta, is encoded by the gene belongs to the cytidylyltransferase family. It is involved in the regulation of phosphatidylcholine biosynthesis. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. PCYT1B encodes three isoforms of CTβ (CTβ1, CTβ2 and CTβ3). CTβ1, CTβ2 isoforms are present in humans and CTβ2 and CTβ3 isoforms have been identified in mice. The CTβ2 can be detected as 41 kDa, and CTβ3 can be detected as 39 kDa. This antibody can recognizes both PCYT1A and PCYT1B. PCYT1A usually exists in the form of a 80 kDa dimer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCYT1B/PCYT1A antibody 13765-1-AP | Download protocol |
| IP protocol for PCYT1B/PCYT1A antibody 13765-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
