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验证数据展示

  • WB analysis using 30991-1-AP (same clone as 30991-1-PBS)

    WB analysis using 30991-1-AP (same clone as 30991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • WB analysis using 30991-1-AP (same clone as 30991-1-PBS)

    WB analysis using 30991-1-AP (same clone as 30991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 30991-1-AP (same clone as 30991-1-PBS)

    IF Staining of A431 using 30991-1-AP (same clone as 30991-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 30991-1-AP (same clone as 30991-1-PBS)

    IF Staining of U-251 using 30991-1-AP (same clone as 30991-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

PCYT1A Polyclonal antibody, PBS Only

PCYT1A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30991-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

CCT A, CCT alpha, CT A, CTPCT, PCYT1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30991-1-PBS targets PCYT1A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34268

Product name: Recombinant human PCYT1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-76 aa of NM_005017

Sequence: MDAQCSAKVNARKRRKEAPGPNGATEEDGVPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEASRGTPCERP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphate cytidylyltransferase 1, choline, alpha
别名 CCT A, CCT alpha, CT A, CTPCT, PCYT1
计算分子量42 kDa
观测分子量40-41 kDa
GenBank蛋白编号NM_005017
基因名称 PCYT1A
Gene ID (NCBI) 5130
RRIDAB_3742383
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP49585
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCYT1A (phosphate cytidylyltransferase 1A, choline), also known as CTPCT. It is expected to be located in the nucleus and cytoplasm, which is enriched in brain, placenta, liver, fetal and adult lung. This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. The molecular weight of PCYT1A is 41 kDa.

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