验证数据展示
经过测试的应用
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18868-1-AP targets PCYOX1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | PCYOX1 fusion protein Ag4316 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | prenylcysteine oxidase 1 |
别名 | KIAA0908, PCL1, PCYOX1, Prenylcysteine lyase, prenylcysteine oxidase 1 |
计算分子量 | 505 aa, 57 kDa |
GenBank蛋白编号 | BC033815 |
基因名称 | PCYOX1 |
Gene ID (NCBI) | 51449 |
RRID | AB_10598471 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UHG3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCYOX1(Prenylcysteine oxidase 1) is also named as KIAA0908, PCL1, prenylcysteine lyase and belongs to the prenylcysteine oxidase family. The deduced 505-amino acid protein contains a predicted signal sequence, several transmembrane regions, and four potential N-glycosylation sites. It is a glycosylated enzyme involved in prenylated protein catabolism, releasing a free cysteine and a hydrophobic isoprenoid product. Immunofluorescence studies of PCYOX1 showed a nonnuclear punctate pattern, and PCYOX1 colocalized with lysosomal marker LAMP1 in HEK293 cells(PMID:10585463). The enzyme is isolated from bovine brain membranes and exhibits an apparent molecular mass of 63 kDa(PMID:9287348).
实验方案
Product Specific Protocols | |
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IHC protocol for PCYOX1 antibody 18868-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |