验证数据展示
经过测试的应用
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18868-1-AP targets PCYOX1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4316 Product name: Recombinant human PCYOX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 155-505 aa of BC033815 Sequence: WVEDVLDKFMRIYRYQSHDYAFSSVEKLLHALGGDDFLGMLNRTLLETLQKAGFSEKFLNEMIAPVMRVNYGQSTDINAFVGAVSLSCSDSGLWAVEGGNKLVCSGLLQASKSNLISGSVMYIEEKTKTKYTGNPTKMYEVVYQIGTETRSDFYDIVLVATPLNRKMSNITFLNFDPPIEEFHQYYQHIVTTLVKGELNTSIFSSRPIDKFGLNTVLTTDNSDLFINSIGIVPSVREKEDPEPSTDGTYVWKIFSQETLTKAQILKLFLSYDYAVKKPWLAYPHYKPPEKCPSIILHDRLYYLNGIECAASAMEMSAIAAHNAALLAYHRWNGHTDMIDQDGLYEKLKTEL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prenylcysteine oxidase 1 |
| 别名 | KIAA0908, PCL1, PCYOX1, Prenylcysteine lyase, prenylcysteine oxidase 1 |
| 计算分子量 | 505 aa, 57 kDa |
| GenBank蛋白编号 | BC033815 |
| 基因名称 | PCYOX1 |
| Gene ID (NCBI) | 51449 |
| RRID | AB_10598471 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHG3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCYOX1(Prenylcysteine oxidase 1) is also named as KIAA0908, PCL1, prenylcysteine lyase and belongs to the prenylcysteine oxidase family. The deduced 505-amino acid protein contains a predicted signal sequence, several transmembrane regions, and four potential N-glycosylation sites. It is a glycosylated enzyme involved in prenylated protein catabolism, releasing a free cysteine and a hydrophobic isoprenoid product. Immunofluorescence studies of PCYOX1 showed a nonnuclear punctate pattern, and PCYOX1 colocalized with lysosomal marker LAMP1 in HEK293 cells(PMID:10585463). The enzyme is isolated from bovine brain membranes and exhibits an apparent molecular mass of 63 kDa(PMID:9287348).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCYOX1 antibody 18868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
