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验证数据展示

  • WB analysis of HEK-293 using 12207-1-AP

    WB analysis of HEK-293 using 12207-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 12207-1-AP

    IHC staining of human ovary cancer using 12207-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12207-1-AP (PCTAIRE3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCTAIRE3 antibody included
  • IF Staining of HEK-293 using 12207-1-AP

    IF Staining of HEK-293 using 12207-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PCTAIRE3 antibody (12207-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

PCTAIRE3 Polyclonal antibody

PCTAIRE3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12207-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CDK18, Cyclin-dependent kinase 18, EC:2.7.11.22, PCTAIRE, PCTAIRE-motif protein kinase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PCTAIRE3 Ready-To-Use IHC Kit. PCTAIRE3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12207-1-AP targets PCTAIRE3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2851

Product name: Recombinant human PCTK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 144-472 aa of BC011526

Sequence: KLDKLGEGTYATVFKGRSKLMENLVALKEIRLEHEEGAPCTAIREVSLLKNLKHANIVTLHDLIHTDRSLTLVFEYLDSDLKQYLDHCGNLMSMHNVKIFMFQLLRGLAYCHHRKILHRDLKPQNLLINERGELKLADFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGSTEYSTPIDMWGVGCIHYEMATGRPLFPGSTVKEELHLIFRLLGTPTEETWPGVTAFSEFRTYSFPCYLPQPLINHAPRLDTDGIHLLSSLLLYESKSRMSAEAALSHSYFRSLGERVHQLEDTASIFSLKEIQLQKDPGYRGLAFQQPGRGKNRRQSIF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PCTAIRE protein kinase 3
别名 CDK18, Cyclin-dependent kinase 18, EC:2.7.11.22, PCTAIRE, PCTAIRE-motif protein kinase 3
计算分子量 472 aa, 54 kDa
观测分子量 54 kDa, 58 kDa
GenBank蛋白编号BC011526
基因名称 PCTAIRE3
Gene ID (NCBI) 5129
RRIDAB_906375
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07002
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCTAIRE3 (PCTAIRE-motif protein kinase 3), also named CDK18 or PCTK3, is a member of PCTAIRE subfamily. The PCTAIRE kinase (PCTK) subfamily of CDKs includes three members, PCTK1/CDK16, PCTK2/CDK17, and PCTK3/CDK18, which contain the PCTAIRE sequence instead of the PSTAIRE sequence (PMID: 24831015). PCTK3 has three isoforms with the molecular mass of 54-58kD.

实验方案

Product Specific Protocols
WB protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
IHC protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
IF protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols