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PCTAIRE3 Polyclonal antibody

PCTAIRE3 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12207-1-AP

Print datasheet

Synonyms

CDK18, PCTAIRE, PCTAIRE motif protein kinase 3, PCTAIRE protein kinase 3, PCTAIRE3, PCTK3

验证数据展示

  • IF Staining of HEK-293 using 12207-1-AP

    IF Staining of HEK-293 using 12207-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 12207-1-AP (PCTAIRE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293 using 12207-1-AP

    WB analysis of HEK-293 using 12207-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12207-1-AP targets PCTAIRE3 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse
Immunogen PCTAIRE3 fusion protein Ag2851 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PCTAIRE protein kinase 3
Synonyms CDK18, PCTAIRE, PCTAIRE motif protein kinase 3, PCTAIRE protein kinase 3, PCTAIRE3, PCTK3
Calculated Molecular Weight 472 aa, 54 kDa
Observed Molecular Weight 54 kDa, 58 kDa
GenBank Accession NumberBC011526
Gene Symbol PCTK3
Gene ID (NCBI) 5129
RRIDAB_906375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07002
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCTAIRE3 (PCTAIRE-motif protein kinase 3), also named CDK18 or PCTK3, is a member of PCTAIRE subfamily. The PCTAIRE kinase (PCTK) subfamily of CDKs includes three members, PCTK1/CDK16, PCTK2/CDK17, and PCTK3/CDK18, which contain the PCTAIRE sequence instead of the PSTAIRE sequence (PMID: 24831015). PCTK3 has three isoforms with the molecular mass of 54-58kD.

实验方案

Product Specific Protocols
WB protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
IF protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols