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验证数据展示

  • WB analysis of human testis using 15106-1-AP

    WB analysis of human testis using 15106-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15106-1-AP (PCSK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 15106-1-AP

    IHC staining of human placenta using 15106-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 15106-1-AP

    IHC staining of human placenta using 15106-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PCSK4 Polyclonal antibody

PCSK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15106-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

PC4, PCSK4, Proprotein convertase 4, SPC5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, human placenta tissue
Positive IHC detected inhuman placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15106-1-AP targets PCSK4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4007

Product name: Recombinant human PCSK4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-242 aa of BC036354

Sequence: MGTRSTLVAIRPLDVSTEGYNNWVFMSTHFWDENPQGVWTLGLENKGYYFNTGTLYRYTLLLYGTAEDMTARPTGPQVTSSACVQRDTEGLCQACDGPAYILGQLCLAYCPPRFFNHTRLVTAGPGHTAAPALRVCSSCHASCYTCRGGSPRDCTSCPPSSTLDQQQGSCMGPTTPDSRPRLRAAACPHHRCPASAMVLSLLAVTLGGPVLCGMSMDLPLYAWLSRARATPTKPQVWLPAGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proprotein convertase subtilisin/kexin type 4
别名 PC4, PCSK4, Proprotein convertase 4, SPC5
计算分子量 83 kDa
观测分子量 54 kDa
GenBank蛋白编号BC036354
基因名称 PCSK4
Gene ID (NCBI) 54760
RRIDAB_10644152
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UW60
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proprotein convertase subtilysin/Kexin Type4 (PCSK4) is a protein convertase enzymes in spermatozoa acrosome membrane, it has an important role in the acrosome reaction through endoproteolytic process that converts the precursor proteins into bioactive protein as a necessary substance to penetrate on the zona pellucida (PMID: 16040806 ). The molecular mass of pro-PCSK4 is 72kDa, and mature PCSK4 is 54 kDa (PMID: 16040806, 19109312).

实验方案

Product Specific Protocols
IHC protocol for PCSK4 antibody 15106-1-APDownload protocol
WB protocol for PCSK4 antibody 15106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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