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  • Featured Product
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PCP4L1 Polyclonal antibody

PCP4L1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25933-1-AP

Print datasheet

Synonyms

IQM1, PCP4 like protein 1, PCP4L1, Purkinje cell protein 4 like 1

验证数据展示

  • WB analysis of mouse brain using 25933-1-AP

    WB analysis of mouse brain using 25933-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25933-1-AP (PCP4L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25933-1-AP

    IHC staining of mouse brain using 25933-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25933-1-AP (PCP4L1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25933-1-AP

    IHC staining of mouse brain using 25933-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25933-1-AP (PCP4L1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25933-1-AP targets PCP4L1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PCP4L1 fusion protein Ag23061 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Purkinje cell protein 4 like 1
Synonyms IQM1, PCP4 like protein 1, PCP4L1, Purkinje cell protein 4 like 1
Calculated Molecular Weight 68 aa, 7 kDa
Observed Molecular Weight 8-15 kDa
GenBank Accession NumberBC028905
Gene Symbol PCP4L1
Gene ID (NCBI) 654790
RRIDAB_2880301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NKN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCP4L1 (Purkinje cell protein 4-like 1) is a small neuronal IQ motif protein closely related to the calmodulin-binding protein Pcp4/PEP-19 (PMID: 22458599).

实验方案

Product Specific Protocols
WB protocol for PCP4L1 antibody 25933-1-APDownload protocol
IHC protocol for PCP4L1 antibody 25933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Genome Biol

A novel antiviral lncRNA, EDAL, shields a T309 O-GlcNAcylation site to promote EZH2 lysosomal degradation.

Authors - Baokun Sui
  • KD Validated
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mouseIHC

J Gen Virol

lncRNA EDAL restricts rabies lyssavirus replication in a cell-specific and infection route-dependent manner.

Authors - Baokun Sui
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mouseWB

J Proteome Res

Multiplexed Quantitative Proteomics Reveals Proteomic Alterations in Two Rodent Traumatic Brain Injury Models

Authors - Junho Park
View Article