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验证数据展示

  • IHC staining of mouse cerebellum using 13774-1-AP

    IHC staining of mouse cerebellum using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 13774-1-AP

    IHC staining of mouse cerebellum using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 13774-1-AP

    IF Staining of mouse cerebellum using 13774-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PCP2 antibody (13774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 13774-1-AP

    IF Staining of mouse cerebellum using 13774-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PCP2 antibody (13774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PCP2 Polyclonal antibody

PCP2 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 13774-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

Purkinje cell protein 2, L7, GPSM4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13774-1-AP targets PCP2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4759

Product name: Recombinant human PCP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-136 aa of BC038715

Sequence: MMDQEEKTEEGSGPCAEAGSPDQEGFFNLLSHVQGDRMEGQRCSLQAGPGQTTKSQSDPTPEMDSLMDMLASTQGRRMDDQRVTVSSLPGFQPVGSKDGAQKRAGTLSPQPLLTPQDPTALGFRRNSSPQPPTQAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Purkinje cell protein 2
别名 Purkinje cell protein 2, L7, GPSM4
计算分子量 136 aa, 15 kDa
GenBank蛋白编号BC038715
基因名称 PCP2
Gene ID (NCBI) 126006
RRIDAB_2935435
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pcp2 is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons, and it may function as a cell-type specific modulator for G protein-mediated cell signaling.

实验方案

Product Specific Protocols
IF protocol for PCP2 antibody 13774-1-APDownload protocol
IHC protocol for PCP2 antibody 13774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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