验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
10607-1-AP targets PCOLCE2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag0879 Product name: Recombinant human PCOLCE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-276 aa of BC006265 Sequence: MRGANAWAPLCLLLAAATQLSRQQSPERPVFTCGGILTGESGFIGSEGFPGVYPPNSKCTWKITVPEGKVVVLNFRFIDLESDNLCRYDFVDVYNGHANGQRIGRFCGTFRPGALVSSGNKMMVQMISDANTAGNGFMAMFSAAEPNERGDQYCGGLLDRPSGSFKTPNWPDRDYPAGVTCVWHIVAPKNQLIELKFEKFDVERDNYCRYDYVAVFNGGEVNDARRIGKYCGDSPPAPIVSERNELLIQFLSDLSLTADGFIGHYIFRPKKLPTTT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | procollagen C-endopeptidase enhancer 2 |
| 别名 | PCOLCE2, PCPE 2, PCPE2 |
| 计算分子量 | 46 kDa |
| GenBank蛋白编号 | BC006265 |
| 基因名称 | PCOLCE2 |
| Gene ID (NCBI) | 26577 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UKZ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Procollagen C-endopeptidase enhancer 2 (PCOLCE2) is a 415-amino acid protein containing an N-terminal signal sequence, two CUB domains, and an NTR domain. PCOLCE2 binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1. It has been reported that PCOLCE2 is a glycoprotein with an apparent molecular weight of 52 kDa, which is larger than its calculated molecular weight of 45.7 kDa (PMID: 10873381).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCOLCE2 antibody 10607-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |