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验证数据展示

  • WB analysis using 11180-2-AP

    WB analysis using 11180-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11180-2-AP (PCNP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11180-2-AP

    IHC staining of human lymphoma using 11180-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11180-2-AP (PCNP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 11180-2-AP

    IHC staining of human lymphoma using 11180-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11180-2-AP (PCNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HepG2 using 11180-2-AP

    IP experiment of HepG2 using 11180-2-AP

    IP result of anti-PCNP (IP:11180-2-AP, 4ug; Detection:11180-2-AP 1:600) with HepG2 cells lysate 1800ug.

  • IF Staining of HeLa using 11180-2-AP

    IF Staining of HeLa using 11180-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCNP antibody (11180-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

PCNP Polyclonal antibody

PCNP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11180-2-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11180-2-AP targets PCNP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag1663

Product name: Recombinant human PCNP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-130 aa of BC013916

Sequence: MADGKAGDEKPEKSQRAGAAGGPEEEAEKPVKTKTVSSSNGGESSSRSAEKRSAEEEAADLPTKPTKISKFGFAIGSQTTKKASAISIKLGSSKPKETVPTLAPKTLSVAAAFNEDEDGYTNISWTKLLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PEST proteolytic signal containing nuclear protein
别名 PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein
计算分子量 19 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC013916
基因名称 PCNP
Gene ID (NCBI) 57092
RRIDAB_2160652
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WW12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEST-containing nuclear protein (PCNP), characterized by a PEST motif rich in proline (P), glutamic acid (E), serine (S), and threonine (T), is firstly identified by database mining. The PCNP protein with the PEST motif is highly subjected to ubiquitination. PCNP may act as a transcriptional factor, cell cycle regulatory protein and tumor regulating nuclear protein.(PMID: 35813472, PMID: 32548978, PMID: 31487123)

实验方案

Product Specific Protocols
IF protocol for PCNP antibody 11180-2-APDownload protocol
IHC protocol for PCNP antibody 11180-2-APDownload protocol
IP protocol for PCNP antibody 11180-2-APDownload protocol
WB protocol for PCNP antibody 11180-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition.

Authors - Pengzhen Dong
View Article
humanWB,IHC,IF

BMC Cancer

PEST-containing nuclear protein mediates the proliferation, migration, and invasion of human neuroblastoma cells through MAPK and PI3K/AKT/mTOR signaling pathways.

Authors - Dong-Dong Wu
  • KD Validated
View Article
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