验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11180-2-AP targets PCNP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | PCNP fusion protein Ag1663 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PEST proteolytic signal containing nuclear protein |
| 别名 | PEST-containing nuclear protein, PEST proteolytic signal-containing nuclear protein |
| 计算分子量 | 19 kDa |
| 观测分子量 | 24-28 kDa |
| GenBank蛋白编号 | BC013916 |
| 基因名称 | PCNP |
| Gene ID (NCBI) | 57092 |
| RRID | AB_2160652 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WW12 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCNP can be detected as 24-28 kDa after posttranslational modification.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCNP antibody 11180-2-AP | Download protocol |
| IHC protocol for PCNP antibody 11180-2-AP | Download protocol |
| IF protocol for PCNP antibody 11180-2-AP | Download protocol |
| IP protocol for PCNP antibody 11180-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition. | ||
BMC Cancer PEST-containing nuclear protein mediates the proliferation, migration, and invasion of human neuroblastoma cells through MAPK and PI3K/AKT/mTOR signaling pathways.
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