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  • Featured Product
  • KD/KO Validated

PCNP Polyclonal antibody

PCNP Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11180-2-AP

Print datasheet

Synonyms

PCNP

验证数据展示

  • WB analysis using 11180-2-AP

    WB analysis using 11180-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11180-2-AP (PCNP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11180-2-AP

    IHC staining of human lymphoma using 11180-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11180-2-AP (PCNP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 11180-2-AP

    IHC staining of human lymphoma using 11180-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11180-2-AP (PCNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HepG2 using 11180-2-AP

    IP experiment of HepG2 using 11180-2-AP

    IP result of anti-PCNP (IP:11180-2-AP, 4ug; Detection:11180-2-AP 1:600) with HepG2 cells lysate 1800ug.

  • IF Staining of HepG2 using 11180-2-AP

    IF Staining of HepG2 using 11180-2-AP

    Immunofluorescent analysis of HepG2 cells using 11180-2-AP (PCNP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11180-2-AP targets PCNP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen PCNP fusion protein Ag1663 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PEST proteolytic signal containing nuclear protein
Synonyms PCNP
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC013916
Gene Symbol PCNP
Gene ID (NCBI) 57092
RRIDAB_2160652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCNP can be detected as 24-28 kDa after posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for PCNP antibody 11180-2-APDownload protocol
IHC protocol for PCNP antibody 11180-2-APDownload protocol
IF protocol for PCNP antibody 11180-2-APDownload protocol
IP protocol for PCNP antibody 11180-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition.

Authors - Pengzhen Dong
View Article
humanWB,IHC,IF

BMC Cancer

PEST-containing nuclear protein mediates the proliferation, migration, and invasion of human neuroblastoma cells through MAPK and PI3K/AKT/mTOR signaling pathways.

Authors - Dong-Dong Wu
  • KD Validated
View Article