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PCM1 Polyclonal antibody
PCM1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HT-1080 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, NIH/3T3 cells, HeLa cells |
| Positive FC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19856-1-AP targets PCM1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | PCM1 fusion protein Ag13929 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | pericentriolar material 1 |
| Synonyms | hPCM 1, PCM 1, PCM1, pericentriolar material 1, PTC4 |
| Calculated Molecular Weight | 2024 aa, 228 kDa |
| Observed Molecular Weight | 260-280 kDa |
| GenBank Accession Number | BC000453 |
| Gene Symbol | PCM1 |
| Gene ID (NCBI) | 5108 |
| RRID | AB_2878616 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15154 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCM1 antibody 19856-1-AP | Download protocol |
| IHC protocol for PCM1 antibody 19856-1-AP | Download protocol |
| IF protocol for PCM1 antibody 19856-1-AP | Download protocol |
| FC protocol for PCM1 antibody 19856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia. | ||
J Cell Biol A ciliopathy complex builds distal appendages to initiate ciliogenesis.
| ||
EMBO Rep Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function. | ||
Cardiovasc Diabetol Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition. |
