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验证数据展示

  • IF Staining of mouse brain using 18156-1-AP

    IF Staining of mouse brain using 18156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Piccolo Polyclonal antibody

Piccolo Polyclonal Antibody for IF-P, ELISA
Cat No. 18156-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P, ELISA

别名

ACZ, Aczonin, KIAA0559, PCLO, Piccolo, Protein piccolo

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18156-1-AP targets Piccolo in IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Piccolo fusion protein Ag12952 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 piccolo (presynaptic cytomatrix protein)
别名 ACZ, Aczonin, KIAA0559, PCLO, Piccolo, Protein piccolo
计算分子量 553 kDa
GenBank蛋白编号BC001304
基因名称 Piccolo
Gene ID (NCBI) 27445
RRIDAB_3085556
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6V0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Piccolo is a presynaptic scaffolding protein of the cytomatrix assembled at the active zone (AZ). AZ is a special region of the presynaptic plasma membrane where synaptic vesicles (SVs) fuse. It has been shown that Piccolo affects presynaptic function by negatively regulating SV exocytosis (PMID: 18519737).

实验方案

Product Specific Protocols
IF protocol for Piccolo antibody 18156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols