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验证数据展示

  • WB analysis using 67676-1-Ig (same clone as 67676-1-PBS)

    WB analysis using 67676-1-Ig (same clone as 67676-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

    IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK2 antibody included
  • IP experiment of HepG2 using 67676-1-Ig (same clone as 67676-1-PBS)

    IP experiment of HepG2 using 67676-1-Ig (same clone as 67676-1-PBS)

    IP result of anti-PCK2 (IP:67676-1-Ig, 4ug; Detection:67676-1-Ig 1:2000) with HepG2 cells lysate 1640 ug. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67676-1-Ig (same clone as 67676-1-PBS)

    IF Staining of MCF-7 using 67676-1-Ig (same clone as 67676-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCK2 antibody (67676-1-Ig, Clone: 1B3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

PCK2 Monoclonal antibody, PBS Only

PCK2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 67676-1-PBS

产品说明书

CloneNo. 1B3F1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, IP, ELISA

别名

1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PCK2 Ready-To-Use IHC Kit. PCK2 primary antibody included.

产品信息

67676-1-PBS targets PCK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 PCK2 fusion protein Ag7021 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoenolpyruvate carboxykinase 2 (mitochondrial)
别名 1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M
计算分子量 71 kDa
观测分子量68 kDa
GenBank蛋白编号BC001454
基因名称 PCK2
Gene ID (NCBI) 5106
RRIDAB_2882871
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16822
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.