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验证数据展示

  • WB analysis using 24103-1-AP (same clone as 24103-1-PBS)

    WB analysis using 24103-1-AP (same clone as 24103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24103-1-AP (PCGF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24103-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 24103-1-AP (same clone as 24103-1-PBS)

    WB analysis of HEK-293 using 24103-1-AP (same clone as 24103-1-PBS)

    WB result of PCGF6 antibody (24103-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCGF6 transfected HEK-293 cells. This data was developed using the same antibody clone with 24103-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 24103-1-AP (same clone as 24103-1-PBS)

    IHC staining of mouse testis using 24103-1-AP (same clone as 24103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24103-1-AP (PCGF6 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24103-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCGF6 antibody included
  • IHC staining of mouse testis using 24103-1-AP (same clone as 24103-1-PBS)

    IHC staining of mouse testis using 24103-1-AP (same clone as 24103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24103-1-AP (PCGF6 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24103-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCGF6 antibody included
  • FC experiment of HEK-293T using 24103-1-AP (same clone as 24103-1-PBS)

    FC experiment of HEK-293T using 24103-1-AP (same clone as 24103-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human PCGF6 (24103-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 24103-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293T using 24103-1-AP (same clone as 24103-1-PBS)

    IP experiment of HEK-293T using 24103-1-AP (same clone as 24103-1-PBS)

    IP result of anti-PCGF6 (IP:24103-1-AP, 4ug; Detection:24103-1-AP 1:1500) with HEK-293T cells lysate 2200 ug. This data was developed using the same antibody clone with 24103-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PCGF6 Polyclonal antibody, PBS Only

PCGF6 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 24103-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

MBLR, Mel18 and Bmi1-like RING finger, PCGF 6, RNF134

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PCGF6 Ready-To-Use IHC Kit. PCGF6 primary antibody included.

产品信息

24103-1-PBS targets PCGF6 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21124

Product name: Recombinant human PCGF6 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-352 aa of BC010235

Sequence: MEGVAVVTAGSVGAAKTEGAAALPPPPPPPVSPPALTPAPAAGEEGPAPLSETGAPGCSGSRPPELEPERSLGRFRGRFEDEDEELEEEEELEEEEEEEEEDMSHFSLRLEGGRQDSEDEEERLINLSELTPYILCSICKGYLIDATTITECLHTFCKSCIVRHFYYSNRCPKCNIVVHQTQPLYNIRLDRQLQDIVYKLVINLEEREKKQMHDFYKERGLEVPKPAVPQPVPSSKGRSKKVLESVFRIPPELDMSLLLEFIGANEGTGHFKPLEKKFVRVSGEATIGHVEKFLRRKMGLDPACQVDIICGDHLLEQYQTLREIRRAIGDAAMQDGLLVLHYGLVVSPLKIT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polycomb group ring finger 6
别名 MBLR, Mel18 and Bmi1-like RING finger, PCGF 6, RNF134
计算分子量 352 aa, 39 kDa
观测分子量40-50 kDa
GenBank蛋白编号BC010235
基因名称 PCGF6
Gene ID (NCBI) 84108
RRIDAB_2737369
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9BYE7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.

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