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验证数据展示

  • IHC staining of mouse brain using 10857-1-AP

    IHC staining of mouse brain using 10857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10857-1-AP (PCDHGC3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10857-1-AP

    IHC staining of mouse brain using 10857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10857-1-AP (PCDHGC3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 10857-1-AP

    IF Staining of SH-SY5Y using 10857-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PCDHGC3 antibody (10857-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PCDHGC3 Polyclonal antibody

PCDHGC3 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 10857-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

PC-43, PCDH2, PCDH-gamma-C3, Protocadherin gamma-C3, Protocadherin-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10857-1-AP targets PCDHGC3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PCDHGC3 fusion protein Ag1243 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin gamma subfamily C, 3
别名 PC-43, PCDH2, PCDH-gamma-C3, Protocadherin gamma-C3, Protocadherin-2
计算分子量 150 kDa
GenBank蛋白编号BC019299
基因名称 PCDHGC3
Gene ID (NCBI) 5098
RRIDAB_2159713
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UN70
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCDHGC3, a member of the PCDHγ cluster, has been described in some studies as a tumor suppressor that promotes the apoptosis of tumor cells, as well as suppresses the Wnt- and mTOR-signaling pathways, which negatively affects the growth of various tumors such as Wilms tumors, breast cancer, or prostate cancer.

实验方案

Product Specific Protocols
IHC protocol for PCDHGC3 antibody 10857-1-APDownload protocol
IF protocol for PCDHGC3 antibody 10857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols