验证数据展示
经过测试的应用
| Positive IP detected in | A375 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17050-1-AP targets PCDHB13 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IP, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag10713 Product name: Recombinant human PCDHB13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-353 aa of BC033068 Sequence: GLSLAGAAEPRSYSVVEETEGSSFVTNLAKDLGLEQREFSRRGVRVVSRGNKLHLQLNQETADLLLNEKLDREDLCGHTEPCVLRFQVLLESPFEFFQAELQVIDINDHSPVFLDKQMLVKVSESSPPGTTFPLKNAEDLDVGQNNIENYIISPNSYFRVLTRKRSDGRKYPELVLDKALDREEEAELRLTLTALDGGSPPRSGTAQVYIEVLDVNDNAPEFEQPFYRVQISEDSPVGFLVVKVSATDVDTGVNGEISYSLFQASEEIGKTFKINPLTGEIELKKQLDFEKLQSYEVNIEARDAGTFSGKCTVLIQVIDVNDHAPEVTMSAF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protocadherin beta 13 |
| 别名 | PCDH BETA13, PCDH-beta-13, Protocadherin beta-13 |
| 计算分子量 | 798 aa, 88 kDa |
| 观测分子量 | 88 kDa |
| GenBank蛋白编号 | BC033068 |
| 基因名称 | PCDHB13 |
| Gene ID (NCBI) | 56123 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5F0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocadherin beta-13 (PCDHB13) is a potential calcium-dependent cell-adhesion protein. PCDHB13 may be involved in the establishment and maintenance of specific neuronal connections in the brain. PCDHB13 is highly expressed on the melanoma cell surface (PMID: 27068704). The calculated molecular weight of PCDHB13 is 88 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCDHB13 antibody 17050-1-AP | Download protocol |
| IP protocol for PCDHB13 antibody 17050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
