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验证数据展示

  • IP experiment of A375 using 17050-1-AP

    IP experiment of A375 using 17050-1-AP

    IP result of anti-PCDHB13 (IP:17050-1-AP, 4ug; Detection:17050-1-AP 1:500) with A375 cells lysate 1440 ug.

  • IHC staining of mouse brain using 17050-1-AP

    IHC staining of mouse brain using 17050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17050-1-AP (PCDHB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PCDHB13 Polyclonal antibody

PCDHB13 Polyclonal Antibody for IHC, IP, ELISA
Cat No. 17050-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IP, ELISA

别名

PCDH BETA13, PCDH-beta-13, Protocadherin beta-13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inA375 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17050-1-AP targets PCDHB13 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 PCDHB13 fusion protein Ag10713 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin beta 13
别名 PCDH BETA13, PCDH-beta-13, Protocadherin beta-13
计算分子量 798 aa, 88 kDa
观测分子量88 kDa
GenBank蛋白编号BC033068
基因名称 PCDHB13
Gene ID (NCBI) 56123
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5F0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protocadherin beta-13 (PCDHB13) is a potential calcium-dependent cell-adhesion protein. PCDHB13 may be involved in the establishment and maintenance of specific neuronal connections in the brain. PCDHB13 is highly expressed on the melanoma cell surface (PMID: 27068704). The calculated molecular weight of PCDHB13 is 88 kDa.

实验方案

Product Specific Protocols
IHC protocol for PCDHB13 antibody 17050-1-APDownload protocol
IP protocol for PCDHB13 antibody 17050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols