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验证数据展示

  • IHC staining of human pancreas cancer using 14785-1-AP

    IHC staining of human pancreas cancer using 14785-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14785-1-AP (PCDHB10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHB10 antibody included
  • IHC staining of human pancreas cancer using 14785-1-AP

    IHC staining of human pancreas cancer using 14785-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14785-1-AP (PCDHB10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHB10 antibody included

PCDHB10 Polyclonal antibody

PCDHB10 Polyclonal Antibody for IHC, ELISA
Cat No. 14785-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

PCDH beta 10, PCDH BETA10, PCDHB10, PCHB10, protocadherin beta 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman pancreas cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PCDHB10 Ready-To-Use IHC Kit. PCDHB10 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14785-1-AP targets PCDHB10 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 PCDHB10 fusion protein Ag6427 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin beta 10
别名 PCDH beta 10, PCDH BETA10, PCDHB10, PCHB10, protocadherin beta 10
计算分子量 88 kDa
GenBank蛋白编号BC031837
基因名称 PCDHB10
Gene ID (NCBI) 56126
RRIDAB_3669193
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UN67
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHB10 belongs to the β-protocadherin (PCDHB) cluster. PCDHB cluster acts as a DNA methylation marker of aggressiveness in neuroblastoma (PMID: 26103900).

实验方案

Product Specific Protocols
IHC protocol for PCDHB10 antibody 14785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols