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PCDHA6 Polyclonal antibody

PCDHA6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12853-1-AP

Print datasheet

Synonyms

CNR2, CNRN2, CNRS2, CRNR2, PCDH alpha 6, PCDH ALPHA6, PCDHA6, protocadherin alpha 6

验证数据展示

  • WB analysis using 12853-1-AP

    WB analysis using 12853-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 12853-1-AP

    IHC staining of mouse cerebellum using 12853-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA6 antibody included
  • IHC staining of mouse cerebellum using 12853-1-AP

    IHC staining of mouse cerebellum using 12853-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA6 antibody included

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue, human gliomas tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PCDHA6 Ready-To-Use IHC Kit. PCDHA6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12853-1-AP targets PCDHA6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen PCDHA6 fusion protein Ag3493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protocadherin alpha 6
Synonyms CNR2, CNRN2, CNRS2, CRNR2, PCDH alpha 6, PCDH ALPHA6, PCDHA6, protocadherin alpha 6
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC036674
Gene Symbol PCDHA6
Gene ID (NCBI) 56142
RRIDAB_10642141
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCDHA6 antibody 12853-1-APDownload protocol
IHC protocol for PCDHA6 antibody 12853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols