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验证数据展示

  • WB analysis using 15270-1-AP

    WB analysis using 15270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 15270-1-AP

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA5 antibody included
  • IHC staining of mouse cerebellum using 15270-1-AP

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA5 antibody included
  • IHC staining of mouse cerebellum using 15270-1-AP

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA5 antibody included

PCDHA5 Polyclonal antibody

PCDHA5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15270-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PCDH ALPHA5, PCDH alpha 5, CRNR6, CNRS6, CNRN6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled mouse brain tissue, human brain tissue, unboiled rat brain tissue
Positive IHC detected inmouse cerebellum tissue, human brain tissue, human gliomas tissue, human lung tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PCDHA5 Ready-To-Use IHC Kit. PCDHA5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15270-1-AP targets PCDHA5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PCDHA5 fusion protein Ag7376 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin alpha 5
别名 PCDH ALPHA5, PCDH alpha 5, CRNR6, CNRS6, CNRN6
计算分子量 102 kDa
观测分子量 102 kDa
GenBank蛋白编号BC033735
基因名称 PCDHA5
Gene ID (NCBI) 56143
RRIDAB_2283415
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5H7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCDHA5 antibody 15270-1-APDownload protocol
IHC protocol for PCDHA5 antibody 15270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols