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验证数据展示

  • WB analysis using 18803-1-AP

    WB analysis using 18803-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 18803-1-AP

    WB analysis using 18803-1-AP

    Calu-3 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 18803-1-AP

    IHC staining of mouse brain using 18803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA3 antibody included
  • IHC staining of mouse brain using 18803-1-AP

    IHC staining of mouse brain using 18803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA3 antibody included

PCDHA3 Polyclonal antibody

PCDHA3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18803-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Protocadherin alpha-3, PCDH-alpha-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalu-3 cells, A549 cells, mouse colon tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PCDHA3 Ready-To-Use IHC Kit. PCDHA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18803-1-AP targets PCDHA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin alpha 3
别名 Protocadherin alpha-3, PCDH-alpha-3
计算分子量 102 kDa
观测分子量 102 kDa
GenBank蛋白编号BC129807
基因名称 PCDHA3
Gene ID (NCBI) 56145
RRIDAB_10596786
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5H8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCDHA3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. The antibody can recognize both the isoforms of PCDHA3.

实验方案

Product Specific Protocols
WB protocol for PCDHA3 antibody 18803-1-APDownload protocol
IHC protocol for PCDHA3 antibody 18803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols