验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, U2OS cells, human brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31671-1-AP targets PCDHA1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag36313 Product name: Recombinant human PCDHA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 130-310 aa of BC166697 Sequence: PPVFRGREQIIFIPESRLLNSRFPIEGAADADIGANALLTYTLSPSDYFSLDVEASDELSKSLWLELRKYLDREETPELHLLLTATDGGKPELQGTVELLITVLDVNDNAPLFDQAVYRVHLLETTANGTLVTTLNASDADEGVNGEVVFSFDSGISRDIQEKFKVDSSSGEIRLIDKLDY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protocadherin alpha 1 |
| 别名 | protocadherin alpha 1, PCDH ALPHA1, PCDH alpha 1 |
| 观测分子量 | 102 kDa |
| GenBank蛋白编号 | BC166697 |
| 基因名称 | PCDHA1 |
| Gene ID (NCBI) | 56147 |
| RRID | AB_3670070 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y5I3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocadherin alpha-1 (PCDHA1) is a potential calcium-dependent cell adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDHA1 antibody 31671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
