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验证数据展示

  • WB analysis using 14000-1-AP

    WB analysis using 14000-1-AP

    mouse brain and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14000-1-AP

    WB analysis using 14000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PCDH8 Polyclonal antibody

PCDH8 Polyclonal Antibody for WB, ELISA
Cat No. 14000-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Protocadherin-8, Arcadlin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, L02 cells, Neuro-2a cells, U-251 cells, U-87 MG cells, unboiled mouse brain

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14000-1-AP targets PCDH8 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5090

Product name: Recombinant human PCDH8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-351 aa of BC036025

Sequence: TVRYSTFEEDAPGTVIGTLAEDLHMKVSGDTSFRLMKQFNSSLLRVREGDGQLTVGDAGLDRERLCGQAPQCVLAFDVVSFSQEQFRLVHVEVEVRDVNDHAPRFPRAQIPVEVSEGAAVGTRIPLEVPVDEDVGANGLQTVRLAEPHSPFRVELQTRADGAQCADLVLLQELDRESQAAYSLELVAQDGGRPPRSATAALSVRVLDANDHSPAFPQGAVAEVELAEDAPVGSLLLDLDAADPDEGPNGDVVFAFGARTPPEARRLFRLDPRSGRLTLAGPVDYERQDTYELDVRAQDRGPGPRAATCKVIVRIRDVNDNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin 8
别名 Protocadherin-8, Arcadlin
计算分子量 1070 aa, 113 kDa
观测分子量104 kDa,113 kDa
GenBank蛋白编号BC036025
基因名称 PCDH8
Gene ID (NCBI) 5100
RRIDAB_3669175
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95206
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protocadherin-8 (PCDH8) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH8 is a tumor suppressor involved in tumorigenesis.

实验方案

Product Specific Protocols
WB protocol for PCDH8 antibody 14000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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