验证数据展示
经过测试的应用
| Positive WB detected in | mouse retina tissue, rat retina tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17424-1-AP targets PCDH21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PCDH21 fusion protein Ag11451 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protocadherin 21 |
| 别名 | Protocadherin-21, PRCAD, Photoreceptor cadherin, KIAA1775, CDHR1 |
| 计算分子量 | 745 aa, 82 kDa |
| 观测分子量 | 110-120 kDa |
| GenBank蛋白编号 | BC038799 |
| 基因名称 | PCDH21 |
| Gene ID (NCBI) | 92211 |
| RRID | AB_3669273 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96JP9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). PCDH21 (protocadherin 21), also known as CDHR1, is a photoreceptor-specific cadherin protein containing six ECs and an intracellular domain. The mutations of PCDH21 cause recessive retinal degeneration and autosomal recessive cone-rod dystrophy in humans (PMID: 16288196; PMID: 20805371). The full length of the PCDH21 protein is 120 kDa, the N-terminal soluble fragment is 95 kDa, and the C-terminal fragment is 25 kDa (PMID: 35066146; PMID: 18654668; PMID: 33964272).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDH21 antibody 17424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |