验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29965-1-AP targets PCDH19 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag32302 Product name: Recombinant human PCDH19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 850-1040 aa of NM_001105243.1 Sequence: HHSFNSQGPQQPDLIINGVPLPETENYSFDSNYVNSRAHLIKSSSTFKDLEGNSLKDSGHEESDQTDSEHDVQRSLYCDTAVNDVLNTSVTSMGSQMPDHDQNEGFHCREECRILGHSDRCWMPRNPMPIRSKSPEHVRNIIALSIEATAADVEAYDDCGPTKRTFATFGKDVSDHPAEERPTLKGKRTVD* 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protocadherin 19 |
| 别名 | EFMR, EIEE9 |
| 计算分子量 | 126 kDa |
| 观测分子量 | 126 kDa |
| GenBank蛋白编号 | NM_001105243.1 |
| 基因名称 | PCDH19 |
| Gene ID (NCBI) | 57526 |
| RRID | AB_3742323 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TAB3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCDH19 is a transmembrane protein and member of the protocadherin family. It is encoded by the X-chromosome and more than 200 mutations have been linked to the neurodevelopmental PCDH-clustering epilepsy (PCDH19-CE) syndrome. PCDH19 is a member of the non-clustered δ2-type protocadherins, has 6 cadherin repeats in the extracellular part, and a cytoplasmic tail containing two conserved domains (CM1 and CM2) (PMID: 36389226).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDH19 antibody 29965-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
