验证数据展示
经过测试的应用
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34123-1-AP targets PCDH18 in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag41285 Product name: Recombinant human PCDH18 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 750-900 aa of BC093815 Sequence: PSRQIHKGDITLVPTINGTLPIRSHHRSSPSSSPTLERGQMGSRQSHNSHQSLNSLVTISSNHVPENFSLELTHATPAVEQVSQLLSMLHQGQYQPRPSFRGNKYSRSYRYALQDMDKFSLKDSGRGDSEAGDSDYDLGRDSPIDRLLGEG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protocadherin 18 |
| 别名 | KIAA1562, PCDH68L, Protocadherin-18 |
| 计算分子量 | 1135 aa, 126 kDa |
| GenBank蛋白编号 | BC093815 |
| 基因名称 | PCDH18 |
| Gene ID (NCBI) | 54510 |
| RRID | AB_3743157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9HCL0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocadherin 18 (PCDH18) is a member of the non-clustered protocadherin subfamily within the cadherin superfamily. Encoded by the PCDH18 gene on chromosome 4q28, it contains six to seven extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail. PCDH18 is expressed in brain, heart, kidney, lung, and trachea. It plays critical roles in cell-cell adhesion, neuronal migration, axon guidance, and synapse formation during development. Emerging evidence links PCDH18 mutations or dysregulation to neurodevelopmental disorders, including autism spectrum disorder and intellectual disability, as well as to certain cancers (PMID: 22450339; PMID: 29075151; PMID: 28588296).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCDH18 antibody 34123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
