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验证数据展示

  • WB analysis using 20719-1-AP (same clone as 20719-1-PBS)

    WB analysis using 20719-1-AP (same clone as 20719-1-PBS)

    mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 20719-1-AP (same clone as 20719-1-PBS)

    IF Staining of HEK-293 using 20719-1-AP (same clone as 20719-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PCDH15 antibody (20719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

PCDH15 Polyclonal antibody, PBS Only

PCDH15 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 20719-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20719-1-PBS targets PCDH15 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin 15
别名
计算分子量 216 kDa
观测分子量120-160 kDa, 50 kDa
GenBank蛋白编号NM_033056
基因名称 PCDH15
Gene ID (NCBI) 65217
RRIDAB_3085617
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QU1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protocadherin-15 (PCDH15) belongs to the cadherin superfamily that mediates calcium-dependent cell-cell adhesion and plays an important role in the maintenance of normal tissue architecture. A homozygous variant in PCDH15 results in Usher syndrome type 1F (USH1F) which leads to congenital hearing loss and syndromicretinitis pigmentosa (PMID: 38014037). Multiple isoforms of PCDH15 have been described, including 216-250 kDa (full-length), 150-180 kDa, 100-120 kDa, 50-65 kDa, and 25-40 kDa (PMID: 22363448; 15928608; 14570705; 23035094; 28883094).

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