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验证数据展示

  • WB analysis using 16141-1-AP

    WB analysis using 16141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16141-1-AP (PCBP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 16141-1-AP

    IF Staining of MCF-7 using 16141-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PCBP3 antibody (16141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PCBP3 Polyclonal antibody

PCBP3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16141-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PCBP3-overlapping transcript 1, PCBP3-overlapping transcript, PCBP3-OT1, PCBP3OT, Alpha-CP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-1080 cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16141-1-AP targets PCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9202

Product name: Recombinant human PCBP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC012061

Sequence: MESKVSEGGLNVTLTIRLLMHGKEVGSIIGKKGETVKKMREESGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQCGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLEAYTIQGQYAIPHPDLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNLMGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANISLAQYLINARLTSEVTGMGTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly(rC) binding protein 3
别名 PCBP3-overlapping transcript 1, PCBP3-overlapping transcript, PCBP3-OT1, PCBP3OT, Alpha-CP3
计算分子量 313 aa, 33 kDa
观测分子量37 kDa
GenBank蛋白编号BC012061
基因名称 PCBP3
Gene ID (NCBI) 54039
RRIDAB_3085481
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57721
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. PCBP3 is broadly expressed in various tissues, including the testis and endometrium. It has been associated with diseases such as dementia.

实验方案

Product Specific Protocols
IF protocol for PCBP3 antibody 16141-1-APDownload protocol
WB protocol for PCBP3 antibody 16141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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