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验证数据展示

  • WB analysis using 15702-1-AP (same clone as 15702-1-PBS)

    WB analysis using 15702-1-AP (same clone as 15702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15702-1-AP

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCBD1 antibody included
  • IHC staining of human liver cancer using 15702-1-AP

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCBD1 antibody included
  • IHC staining of human liver cancer using 15702-1-AP

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCBD1 antibody included
  • IHC staining of human liver cancer using 15702-1-AP

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCBD1 antibody included
  • IHC staining of mouse liver using 15702-1-AP (same clone as 15702-1-PBS)

    IHC staining of mouse liver using 15702-1-AP (same clone as 15702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCBD1 antibody included
  • IF Staining of HeLa using 15702-1-AP (same clone as 15702-1-PBS)

    IF Staining of HeLa using 15702-1-AP (same clone as 15702-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCBD1 antibody (15702-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

PCBD1 Polyclonal antibody, PBS Only

PCBD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15702-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

4-alpha-hydroxy-tetrahydropterin dehydratase, DCOH, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, EC:4.2.1.96, PCBD

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PCBD1 Ready-To-Use IHC Kit. PCBD1 primary antibody included.

产品信息

15702-1-PBS targets PCBD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8331

Product name: Recombinant human PCBD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-104 aa of BC006324

Sequence: MAGKAHRLSAEERDQLLPNLRAVGWNELEGRDAIFKQFHFKDFNRAFGFMTRVALQAEKLDHHPEWFNVYNKVHITLSTHECAGLSERDINLASFIEQVAVSMT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
别名 4-alpha-hydroxy-tetrahydropterin dehydratase, DCOH, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, EC:4.2.1.96, PCBD
计算分子量 104 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC006324
基因名称 PCBD1
Gene ID (NCBI) 5092
RRIDAB_2267945
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61457
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCBD1, also known as DCOH and PCBD, belongs to the pterin-4-alpha-carbinolamine dehydratase family. PCBD1 is a bifunctional protein that acts as an enzyme in the regeneration of cofactor tetrahydrobiopterin (BH4), crucial for the function of aromatic amino acid hydroxylases, and as a dimerization cofactor of transcription factors HNF1A and HNF1B, important in liver and pancreas development and function. PCBD1 is expressed in the developing pancreas. The calculated molecular weight of PCBD1 is 12 kDa (PMID: 24204001, 24848070).

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