验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, mouse retina tissue, rat eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31187-1-AP targets PCARE in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | PCARE fusion protein Ag34774 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 2 open reading frame 71 |
| 别名 | C2orf71, FLJ34931 |
| 计算分子量 | 140 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | NM_001029883 |
| 基因名称 | PCARE |
| Gene ID (NCBI) | 388939 |
| RRID | AB_3669889 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | A6NGG8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C2orf71/PCARE (photoreceptor cilium actin regulator) is a retina-specific protein consisting of 1289 amino acids with a predicted molecular weight of 140 kDa. PCARE is predicted to be myristoylated and palmitoylated at its N terminus and plays an important role in delivering actin-associated components to the base of the photoreceptor OSs to regulate the initial development of OS disks (PMID: 32312818, 35253837).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCARE antibody 31187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |