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验证数据展示

  • WB analysis of HeLa using 86504-3-RR (same clone as 86504-3-PBS)

    WB analysis of HeLa using 86504-3-RR (same clone as 86504-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 86504-3-RR (PBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86504-3-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 86504-3-RR (same clone as 86504-3-PBS)

    IHC staining of human stomach using 86504-3-RR (same clone as 86504-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 86504-3-RR (PBX1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86504-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PBX1 antibody included
  • IF Staining of HeLa using 86504-3-RR (same clone as 86504-3-PBS)

    IF Staining of HeLa using 86504-3-RR (same clone as 86504-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBX1 antibody (86504-3-RR, Clone: 251373B4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86504-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86504-3-RR

    Affinity and Kinetic Characterization of 86504-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86504-3-RR against Human PBX1 were performed. The affinity constant is 0.342 nM.

PBX1 Recombinant monoclonal antibody, PBS Only

PBX1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86504-3-PBS

产品说明书

CloneNo. 251373B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Homeobox protein PBX1, Homeobox protein PRL, pre B cell leukemia homeobox 1, Pre-B-cell leukemia transcription factor 1, PRL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PBX1 Ready-To-Use IHC Kit. PBX1 primary antibody included.

产品信息

86504-3-PBS targets PBX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12817

Product name: Recombinant human PBX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC101578

Sequence: MDEQPRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQEEANIYAAKTAVTATNVSAHGSQANSPSTPNSAGSSSSFNMSNSGDLFMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pre-B-cell leukemia homeobox 1
别名 Homeobox protein PBX1, Homeobox protein PRL, pre B cell leukemia homeobox 1, Pre-B-cell leukemia transcription factor 1, PRL
计算分子量 430 aa, 47 kDa
观测分子量 38 kDa, 47 kDa
GenBank蛋白编号BC101578
基因名称 PBX1
Gene ID (NCBI) 5087
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP40424
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PBX1, also named as Pre-B-cell leukemia transcription factor 1, is a 430 amino acid protein, which belongs to the TALE/PBX homeobox family. PBX1 is expressed in all tissues except in cells of the B and T lineage. PBX1 acts as a transcriptional activator of PF4 in complex with MEIS1. PBX1 may have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b: MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer. PBX1 exists two isoforms (PBX1a and PBX1b), and molecular weight of PBX1 is 47 kDa and 38 kDa.

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