PBRM1 Polyclonal antibody
PBRM1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy PBRM1 Ready-To-Use IHC Kit. PBRM1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IP | See 1 publications below |
产品信息
12563-1-AP targets PBRM1 in WB, IP, ELISA, Dot blot applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB, IP, Dot blot |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | PBRM1 fusion protein Ag3148 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | polybromo 1 |
| Synonyms | BAF180, BRG1 associated factor 180, hPB1, PB1, PBRM1, polybromo 1, Polybromo 1D, Protein polybromo 1 |
| Calculated Molecular Weight | 180 kDa |
| Observed Molecular Weight | 180-195 kDa, 100 kDa |
| GenBank Accession Number | BC015323 |
| Gene Symbol | PBRM1 |
| Gene ID (NCBI) | 55193 |
| RRID | AB_2877865 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86U86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PBRM1 antibody 12563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Protein Expr Purif Heterologous expression of the human polybromo-1 protein in the methylotrophic yeast Pichia pastoris. | ||
Cell Death Dis The RBPJ/DAPK3/UBE3A signaling axis induces PBRM1 degradation to modulate the sensitivity of renal cell carcinoma to CDK4/6 inhibitors. | ||
J Am Heart Assoc Aging-Associated ALKBH5-m6A Modification Exacerbates Doxorubicin-Induced Cardiomyocyte Apoptosis Via AT-Rich Interaction Domain 2 |