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验证数据展示

  • WB analysis using 67529-1-Ig (same clone as 67529-1-PBS)

    WB analysis using 67529-1-Ig (same clone as 67529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

    IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX6 antibody included
  • IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

    IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX6 antibody included

PAX6 Monoclonal antibody, PBS Only

PAX6 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67529-1-PBS

产品说明书

CloneNo. 2C5A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA and More (1)

别名

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PAX6 Ready-To-Use IHC Kit. PAX6 primary antibody included.

产品信息

67529-1-PBS targets PAX6 in WB, IHC, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 PAX6 fusion protein Ag29977 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 paired box 6
别名 Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC011953
基因名称 PAX6
Gene ID (NCBI) 5080
RRIDAB_2882748
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26367
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.