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验证数据展示

  • WB analysis using 21631-1-AP

    WB analysis using 21631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21631-1-AP (PATL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 21631-1-AP

    WB analysis of HeLa using 21631-1-AP

    WB result of PATL1 antibody (21631-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PATL1 transfected HeLa cells.

  • IP experiment of K-562 using 21631-1-AP

    IP experiment of K-562 using 21631-1-AP

    IP result of anti-PATL1 (IP:21631-1-AP, 4ug; Detection:21631-1-AP 1:300) with K-562 cells lysate 3320ug.

  • Featured Product
  • KD/KO验证

PATL1 Polyclonal antibody

PATL1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 21631-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

PAT1 like protein 1, patellin1, PATL1, Protein PAT1 homolog 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, K-562 cells
Positive IP detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21631-1-AP targets PATL1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16247

Product name: Recombinant human PATL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 278-627 aa of BC109038

Sequence: LQSTDPYLDDFYYQNYFEKLEKLSAAEEIQGDGPKKERTKLITPQVAKLEHAYKPVQFEGSLGKLTVSSVNNPRKMIDAVVTSRSEDDETKEKQVRDKRRKTLVIIEKTYSLLLDVEDYERRYLLSLEEERPALMDDRKHKICSMYDNLRGKLPGQERPSDDHFVQIMCIRKGKRMVARILPFLSTEQAADILMTTARNLPFLIKKDAQDEVLPCLLSPFSLLLYHLPSVSITSLLRQLMNLPQSAATPALSNPHLTAVLQNKFGLSLLLILLSRGEDLQSSDPATESTQNNQWTEVMFMATRELLRIPQAALAKPISIPTNLVSLFSRYVDRQKLNLLETKLQLVQGIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein associated with topoisomerase II homolog 1 (yeast)
别名 PAT1 like protein 1, patellin1, PATL1, Protein PAT1 homolog 1
计算分子量 770 aa, 87 kDa
观测分子量87 kDa
GenBank蛋白编号BC109038
基因名称 PATL1
Gene ID (NCBI) 219988
RRIDAB_2935454
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86TB9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Patellin1 (PATL1) is a human homolog to yeast P body component Pat1, localizes to P bodies and is required for P body formation. Furthermore, Pat1 is the only decapping activator protein that, besides interacting with deadenylated mRNAs, also binds to eIF4G-, eIF4E- and Pab1p-associated polyadenylated mRNA and locates to polysomes.

实验方案

Product Specific Protocols
IP protocol for PATL1 antibody 21631-1-APDownload protocol
WB protocol for PATL1 antibody 21631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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