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验证数据展示

  • WB analysis of HEK-293 using 14396-1-AP (same clone as 14396-1-PBS)

    WB analysis of HEK-293 using 14396-1-AP (same clone as 14396-1-PBS)

    WB result of PASK antibody (14396-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PASK transfected HEK-293 cells. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • WB analysis using 14396-1-AP (same clone as 14396-1-PBS)

    WB analysis using 14396-1-AP (same clone as 14396-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 14396-1-AP (same clone as 14396-1-PBS)

    IP experiment of K-562 using 14396-1-AP (same clone as 14396-1-PBS)

    IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

    IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

    IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14396-1-AP (same clone as 14396-1-PBS)

    IF Staining of HeLa using 14396-1-AP (same clone as 14396-1-PBS)

    Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PASK Polyclonal antibody, PBS Only

PASK Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14396-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14396-1-PBS targets PASK in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5893

Product name: Recombinant human PASK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 999-1323 aa of BC063585

Sequence: YSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRLLTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PAS domain containing serine/threonine kinase
别名
计算分子量 143 kDa
观测分子量 180 kDa
GenBank蛋白编号BC063585
基因名称 PASK
Gene ID (NCBI) 23178
RRIDAB_2878053
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RG2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAS (Per-Arnt-Sim) domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PAS domain-containing serine/threonine-protein kinase (PASK, also termed PAS-kinase or PASKIN) is an evolutionarily conserved nutrient responsive protein kinase. PASK links energy flux and protein synthesis in yeast and regulates glycogen synthesis in mammals. It has also been implicated in glucose-stimulated INS production in pancreatic beta-cells.

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