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  • Featured Product
  • KD/KO Validated

PASK Polyclonal antibody

PASK Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14396-1-AP

Print datasheet

Synonyms

hPASK, KIAA0135, PAS kinase, PASK, PASKIN, STK37

验证数据展示

  • WB analysis of HEK-293 using 14396-1-AP

    WB analysis of HEK-293 using 14396-1-AP

    WB result of PASK antibody (14396-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PASK transfected HEK-293 cells.

  • WB analysis using 14396-1-AP

    WB analysis using 14396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 14396-1-AP

    IP experiment of K-562 using 14396-1-AP

    IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug.

  • IHC staining of mouse testis using 14396-1-AP

    IHC staining of mouse testis using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 14396-1-AP

    IHC staining of mouse testis using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14396-1-AP

    IF Staining of HeLa using 14396-1-AP

    Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue, human prostate hyperplasia tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14396-1-AP targets PASK in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen PASK fusion protein Ag5893 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PAS domain containing serine/threonine kinase
Synonyms hPASK, KIAA0135, PAS kinase, PASK, PASKIN, STK37
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC063585
Gene Symbol PASK
Gene ID (NCBI) 23178
RRIDAB_2878053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAS (Per-Arnt-Sim) domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PAS domain-containing serine/threonine-protein kinase (PASK, also termed PAS-kinase or PASKIN) is an evolutionarily conserved nutrient responsive protein kinase. PASK links energy flux and protein synthesis in yeast and regulates glycogen synthesis in mammals. It has also been implicated in glucose-stimulated INS production in pancreatic beta-cells.

实验方案

Product Specific Protocols
WB protocol for PASK antibody 14396-1-APDownload protocol
IHC protocol for PASK antibody 14396-1-APDownload protocol
IF protocol for PASK antibody 14396-1-APDownload protocol
IP protocol for PASK antibody 14396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols