验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse heart tissue, mouse spleen tissue, rat heart tissue |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15864-1-AP targets PARS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8413 Product name: Recombinant human PARS2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 126-475 aa of BC007956 Sequence: ELWQATNRWDLMGKELLRLRDRHGKEYCLGPTHEEAITALIASQKKLSYKQLPFLLYQVTRKFRDEPRPRFGLLRGREFYMKDMYTFDSSPEAAQQTYSLVCDAYCSLFNKLGLPFVKVQADVGTIGGTVSHEFQLPVDIGEDRLAICPRCSFSANMETLDLSQMNCPACQGPLTKTKGIEVGHTFYLGTKYSSIFNAQFTNVCGKPTLAEMGCYGLGVTRILAAAIEVLSTEDCVRWPSLLAPYQACLIPPKKGSKEQAASELIGQLYDHITEAVPQLHGEVLLDDRTHLTIGNRLKDANKFGYPFVIIAGKRALEDPAHFEVWCQNTGEVAFLTKDGVMDLLTPVQTV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prolyl-tRNA synthetase 2, mitochondrial (putative) |
| 别名 | EC:6.1.1.15, MT PRORS, Probable proline--tRNA ligase, mitochondrial, Proline tRNA ligase, Prolyl-tRNA synthetase |
| 计算分子量 | 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC007956 |
| 基因名称 | PARS2 |
| Gene ID (NCBI) | 25973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L3T8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Prolyl-tRNA synthetase 2, mitochondrial (PARS2) is a nuclear-encoded mitochondrial aminoacyl-tRNA synthetase (mt-aaRS) responsible for catalyzing the attachment of proline to mitochondrial tRNAPro, an essential step in mitochondrial protein translation. Following cytoplasmic synthesis, PARS2 is imported into the mitochondrial matrix, where it ensures the accurate synthesis of the 13 oxidative phosphorylation (OXPHOS) proteins encoded by mitochondrial DNA. By maintaining the fidelity of mitochondrial translation, PARS2 is indispensable for mitochondrial respiratory chain function, ATP production, and cellular energy metabolism.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PARS2 antibody 15864-1-AP | Download protocol |
| WB protocol for PARS2 antibody 15864-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
