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验证数据展示

  • WB analysis using 17535-1-AP (same clone as 17535-1-PBS)

    WB analysis using 17535-1-AP (same clone as 17535-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17535-1-AP (PARP9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 17535-1-AP (same clone as 17535-1-PBS)

    IF Staining of THP-1 using 17535-1-AP (same clone as 17535-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PARP9 antibody (17535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 17535-1-AP (same clone as 17535-1-PBS)

    IP experiment of MCF-7 using 17535-1-AP (same clone as 17535-1-PBS)

    IP result of anti-PARP9 (IP:17535-1-AP, 4ug; Detection:17535-1-AP 1:3000) with MCF-7 cells lysate 1400 ug. This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17535-1-AP (same clone as 17535-1-PBS)

    IHC staining of mouse brain using 17535-1-AP (same clone as 17535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17535-1-AP (PARP9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17535-1-AP (same clone as 17535-1-PBS)

    IHC staining of mouse brain using 17535-1-AP (same clone as 17535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17535-1-AP (PARP9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PARP9 Polyclonal antibody, PBS Only

PARP9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17535-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ADP-ribosyltransferase diphtheria toxin-like 9, ARTD9, BAL, BAL1, EC:2.4.2.-

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17535-1-PBS targets PARP9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag11587

Product name: Recombinant human PARP9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 476-819 aa of BC039580

Sequence: EARSPAINLMGFNVEEMYEAHAWIQRILSLQNHHIIENNHILYLGRKEHDILSQLQKTSSVSITEIISPGRTELEIEGARADLIEVVMNIEDMLCKVQEEMARKKERGLWRSLGQWTIQQQKTQDEMKENIIFLKCPVPPTQELLDQKKQFEKCGLQVLKVEKIDNEVLMAAFQRKKKMMEEKLHRQPVSHRLFQQVPYQFCNVVCRVGFQRMYSTPCDPKYGAGIYFTKNLKNLAEKAKKISAADKLIYVFEAEVLTGFFCQGHPLNIVPPPLSPGAIDGHDSVVDNVSSPETFVIFSGMQAIPQYLWTCTQEYVQSQDYSSGPMRPFAQHPWRGFASGSPVD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase family, member 9
别名 ADP-ribosyltransferase diphtheria toxin-like 9, ARTD9, BAL, BAL1, EC:2.4.2.-
计算分子量 819 aa, 92 kDa
观测分子量 88 kDa
GenBank蛋白编号BC039580
基因名称 PARP9
Gene ID (NCBI) 83666
RRIDAB_2158929
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IXQ6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by IFNγ, inducing the transcription of IFNγ-controlled genes.

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