验证数据展示
经过测试的应用
| Positive IP detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17754-1-AP targets PARP8 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PARP8 fusion protein Ag12111 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | poly (ADP-ribose) polymerase family, member 8 |
| 别名 | Poly [ADP ribose] polymerase 8, PARP-8, PARP 8, EC:2.4.2.-, ARTD16 |
| 计算分子量 | 812 aa, 91 kDa |
| 观测分子量 | 91-96 kDa |
| GenBank蛋白编号 | BC075801 |
| 基因名称 | PARP8 |
| Gene ID (NCBI) | 79668 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N3A8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein mono-ADP-ribosyltransferase PARP8, also known as ADP-ribosyltransferase diphtheria toxin-like 16 (ARTD16), is primarily localised on the nuclear envelope for the majority of the cell cycle but localises to centrosomes and spindle poles during mitosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for PARP8 antibody 17754-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |