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验证数据展示

  • WB analysis using 55149-1-AP (same clone as 55149-1-PBS)

    WB analysis using 55149-1-AP (same clone as 55149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55149-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 55149-1-AP (same clone as 55149-1-PBS)

    IF Staining of U2OS using 55149-1-AP (same clone as 55149-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55149-1-AP (PARP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 55149-1-AP (same clone as 55149-1-PBS)

    IHC staining of human kidney using 55149-1-AP (same clone as 55149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55149-1-AP (PARP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 55149-1-AP (same clone as 55149-1-PBS)

    IHC staining of mouse testis using 55149-1-AP (same clone as 55149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55149-1-AP (PARP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

PARP2 Polyclonal antibody, PBS Only

PARP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55149-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2, EC:2.4.2.-

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55149-1-PBS targets PARP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase 2
别名 ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2, EC:2.4.2.-
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号NM_005484
基因名称 PARP2
Gene ID (NCBI) 10038
RRIDAB_10858796
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGN5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).

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