验证数据展示
经过测试的应用
| Positive IHC detected in | mouse brain tissue, mouse testis tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20555-1-AP targets PARP2 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | poly (ADP-ribose) polymerase 2 |
| 别名 | ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2, EC:2.4.2.- |
| 计算分子量 | 66 kDa |
| 观测分子量 | 60-66 kDa |
| GenBank蛋白编号 | NM_005484 |
| 基因名称 | PARP2 |
| Gene ID (NCBI) | 10038 |
| RRID | AB_10694417 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UGN5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PRP2 catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. The antibody is specific to PARP2 (C-terminal). PARP2 can be detected as about 62 kDa (PMID: 10364231).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PARP2 antibody 20555-1-AP | Download protocol |
| WB protocol for PARP2 antibody 20555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
