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验证数据展示

  • WB analysis using 20555-1-AP

    WB analysis using 20555-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20555-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20555-1-AP

    IHC staining of mouse brain using 20555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PARP2 Polyclonal antibody

PARP2 Polyclonal Antibody for IHC, ELISA
Cat No. 20555-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

ADPRT2, ADPRT-2, ADPRTL2, ARTD2, hPARP-2, pADPRT-2, PARP2, PARP-2, Poly [ADP-ribose] polymerase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20555-1-AP targets PARP2 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase 2
别名 ADPRT2, ADPRT-2, ADPRTL2, ARTD2, hPARP-2, pADPRT-2, PARP2, PARP-2, Poly [ADP-ribose] polymerase 2
计算分子量 66 kDa
观测分子量60-66 kDa
GenBank蛋白编号NM_005484
基因名称 PARP2
Gene ID (NCBI) 10038
RRIDAB_10694417
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGN5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PRP2 catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. The antibody is specific to PARP2 (C-terminal). PARP2 can be detected as about 62 kDa (PMID: 10364231).

实验方案

Product Specific Protocols
WB protocol for PARP2 antibody 20555-1-APDownload protocol
IHC protocol for PARP2 antibody 20555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols