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验证数据展示

  • WB analysis of Jurkat using 26072-1-AP

    WB analysis of Jurkat using 26072-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 26072-1-AP

    IP experiment of Jurkat using 26072-1-AP

    IP result of anti-PARP10 (IP:26072-1-AP, 4ug; Detection:26072-1-AP 1:500) with Jurkat cells lysate 3200ug.

  • IF Staining of MCF-7 using 26072-1-AP

    IF Staining of MCF-7 using 26072-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PARP10 antibody (26072-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP10 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP10 antibody included

PARP10 Polyclonal antibody

PARP10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26072-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

ADP-ribosyltransferase diphtheria toxin-like 10, ARTD10, EC:2.4.2.-, PARP 10, PARP-10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PARP10 Ready-To-Use IHC Kit. PARP10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26072-1-AP targets PARP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 PARP10 fusion protein Ag23457 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase family, member 10
别名 ADP-ribosyltransferase diphtheria toxin-like 10, ARTD10, EC:2.4.2.-, PARP 10, PARP-10
观测分子量 130-140 kDa
GenBank蛋白编号BC136542
基因名称 PARP10
Gene ID (NCBI) 84875
RRIDAB_2880362
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53GL7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP10 (Poly(ADP-ribose) polymerase family member 10) is a mono-ADP-ribosyltransferase that belongs to the PARP family. It regulates a variety of cellular functions, including gene transcription, DNA repair, and cell cycle control, by catalyzing ADP-ribosylation reactions. PARP10 is distributed in both the nucleus and cytoplasm, participating in the cellular response to DNA damage. In particular, its expression level significantly increases in response to UV-induced DNA damage. Additionally, PARP10 plays a crucial role in the antiviral response, being highly upregulated by interferon (IFN) to inhibit the replication of certain viruses and block protein translation during viral replication. PARP10 is also involved in immune regulation, interacting with NEMO to block the NF-κB signaling pathway and the production of pro-inflammatory cytokines.

实验方案

Product Specific Protocols
WB protocol for PARP10 antibody 26072-1-APDownload protocol
IHC protocol for PARP10 antibody 26072-1-APDownload protocol
IF protocol for PARP10 antibody 26072-1-APDownload protocol
IP protocol for PARP10 antibody 26072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols