PARG Polyclonal antibody

PARG Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27808-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse and More (2)

应用

WB, IP, IHC, ELISA and More (1)

PARG, PARG99

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27808-1-AP targets PARG in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human, Mouse
文献引用反应性human, zebrafish
免疫原 PARG fusion protein Ag27137 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) glycohydrolase
别名 PARG, PARG99
计算分子量 111 kDa
观测分子量 100-111 kDa
GenBank蛋白编号BC052966
基因名称 PARG
Gene ID (NCBI) 8505
RRIDAB_2880979
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86W56
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PARG antibody 27808-1-APDownload protocol
IHC protocol for PARG antibody 27808-1-APDownload protocol
IP protocol for PARG antibody 27808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma.

Authors - Binghai Zhou
zebrafishWB

Int J Mol Sci

Synthesis and Degradation of Poly(ADP-ribose) in Zebrafish Brain Exposed to Aluminum

Authors - Anna Rita Bianchi
humanWB

Onco Targets Ther

Synergistic Effect of Ubiquitin-Specific Protease 14 and Poly(ADP-Ribose) Glycohydrolase Co-Inhibition in BRCA1-Mutant, Poly(ADP-Ribose) Polymerase Inhibitor-Resistant Triple-Negative Breast Cancer Cells

Authors - Pisong Li
humanWB,IF

Viruses

Role of PARP-1 in Human Cytomegalovirus Infection and Functional Partners Encoded by This Virus

Authors - Wenchang Zhang
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