验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HeLa cells, HEK-293 cells, A431 cells, HepG2 cells |
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66968-1-Ig targets PARD3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | Human |
免疫原 | PARD3 fusion protein Ag7656 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | par-3 partitioning defective 3 homolog (C. elegans) |
别名 | ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2 |
计算分子量 | 151 kDa |
观测分子量 | 170 kDa |
GenBank蛋白编号 | BC011711 |
基因名称 | PARD3 |
Gene ID (NCBI) | 56288 |
RRID | AB_2882290 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q8TEW0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PARD3 antibody 66968-1-Ig | Download protocol |
IF protocol for PARD3 antibody 66968-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |